• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >Expression, purification, crystallization and preliminary X-ray crystallographic analysis of human histidine triad nucleotide-binding protein 2 (hHINT2)
【2h】

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of human histidine triad nucleotide-binding protein 2 (hHINT2)

机译：人组氨酸三联体核苷酸结合蛋白2（hHINT2）的表达，纯化，结晶和初步X射线晶体学分析

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Histidine triad nucleotide-binding protein 2 (HINT2) is a mitochondrial adenosine phosphoramidase mainly expressed in the pancreas, liver and adrenal gland. HINT2 possibly plays a role in apoptosis, as well as being involved in steroid biosynthesis, hepatic lipid metabolism and regulation of hepatic mitochondria function. The expression level of HINT2 is significantly down-regulated in hepatocellular carcinoma patients. To date, endogenous substrates for this enzyme, as well as the three-dimensional structure of human HINT2, are unknown. In this study, human HINT2 was cloned, overexpressed in Escherichia coli and purified. Crystallization was performed at 278 K using PEG 4000 as the main precipitant; the crystals, which belonged to the tetragonal space group P41212 with unit-cell parameters a = b = 76.38, c = 133.25 Å, diffracted to 2.83 Å resolution. Assuming two molecules in the asymmetric unit, the Matthews coefficient and the solvent content were calculated to be 2.63 Å(3) Da(-1) and 53.27%, respectively.
机译：组氨酸三联体核苷酸结合蛋白2（HINT2）是一种线粒体腺苷磷酸酰胺酶，主要表达于胰腺，肝脏和肾上腺。 HINT2可能在细胞凋亡中起作用，并参与类固醇的生物合成，肝脂质代谢和肝线粒体功能的调节。 HINT2的表达水平在肝细胞癌患者中显着下调。迄今为止，该酶的内源底物以及人HINT2的三维结构尚不清楚。在这项研究中，人类HINT2被克隆，在大肠杆菌中过表达并纯化。使用PEG 4000作为主要沉淀剂，在278 K下进行结晶。属于四方空间群P41212的晶体，其晶胞参数a = b = 76.38，c = 133.25Å，衍射至2.83Å分辨率。假设在不对称单元中有两个分子，则马修斯系数和溶剂含量分别计算为2.63Å（3）Da（-1）和53.27％。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号